ABSTRACT Coccidiosis, caused by Eimeria species is common in 2–4 month old goats, are highly pathogenic in goat kids. The most common clinical findings are dehydration, weakness, weight loss, diarrhea and anemia. The aim of this study is to determinate of tear secretion in goats kids with coccidiosis and healthy ones by utilizing STT. A total of 20 pure hair goats kids aged between 2-4 months, among which 10 were with clinical coccidiosis (group 1, n=10) and 10 were in the healthy conditions (group 2, n=10), in a single farm located at Siirt, Turkey. Fecal samples were examined microscopically for concentrated flotation and the presence of oocysts. STT test was performed on both eyes of healthy and clinical coccidiosis and results were recorded. group 1 Mean ± SD STT values for right and left eye were 5.8 ± 1.75 and 5.1 ± 1.66 mm/min and group 2 Mean ± SD STT values for right and left eye were 13.4 ± 2.37 and 12.5 ± 2.12 mm/min, respectively. Comparison of STT values of the right eyes with left eyes did not differ significantly in both groups (P = 0.271, P = 0.235). Both eye’s STT results combined to compare groups. The mean of group 1 (5.45 ± 1.7 mm/min) was found to be significantly lower than the mean of group 2 (12.95 ± 2.24 mm/min) as a result (P < 0.001). As a result of this study, the amount of tear secretion was determined with the STT and there was statistically significant difference between healthy and goat kids clinical coccidiosis.
NUTRITIVE VALUE AND CHEMICAL QUALITY INDICATORS OF IMPORTED CATTLE’S LIVER / القيمة الغذائية ومؤشرات الجودة الكيميائية لكبد الأبقار المستورد
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 08/03/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 14
اسماء رفعت احمد ابراهيم
حسين يوسف أحمد( yossefh46@yahoo.com - )
شريف محمد سيد
ضياء بخيت محمد - مؤلف رئيسي
Keywords: Imported frozen liver, Chemical quality, Nutritional aspects
ABSTRACT The present study was conducted to assess the quality of imported frozen liver sold in Assiut markets, Egypt. A total of 100 samples were randomly collected over a 2 months period (January to March, 2020) from poultry slaughter shops, supermarkets and frozen meat markets. The liver samples were evaluated for chemical indicators of spoilage (pH, TVBN ”Total Volatile base nitrogen”, and TBA ”Thiobarbituric acid”) and some of the nutritional aspects (percentage of moisture, protein, fat, ash, and carbohydrates, as well as, gross energy (Kcal/100g) and cholesterol content (mg/100g), beside levels of iron (mg/100g). The obtained mean values of pH, TVBN (mg/100 g) and TBA (mg/kg) of the examined samples were 6.38 ± 0.01, 25.76 ± 0.44, and 0.65 ± 0.03, respectively. Of the examined samples, 92 % showed pH value exceeded the permissible limits of Egyptian standards; however, 88 % showed TVBN content within the set limit. The mean values of moisture, protein, fat, ash and carbohydrates (%) were 70.54 ± 0.11, 21.64 ± 0.08, 3.58± 0.12, 1.50 ± 0.018, and 2.74 ± 0.11, respectively. The calculated gross energy mean value was of 133.09 ± 1.06 Kcal/100 g. Additionally, the cholesterol and iron content mean values were 130.85 ± 2.17 mg/100 g and 16.07 ± 0.24 mg/100 g, respectively in the examined imported frozen liver samples. In conclusion, imported frozen liver sold in markets of Assiut city Egypt is of fair quality; it should be consumed sporadically and with care of TBARS, cholesterol and iron potential hazards.
DETECTION OF MULTI DRUG RESISTANT FOOD BORN BACTERIA IN READY TO EAT CHICKEN MEAT / الكشف عن البكتيريا المقاومة للمضادات الحيوية في لحوم الدواجن الجاهزة للاستهلاك
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 10
اسماء رفعت احمد ابراهيم
زينب احمد محمد( zeinabrenad@gmail.com - ) - مؤلف رئيسي
شيماء النجار طاهر( zeinabrenad@gmail.com - )
Key words: Multi Drug resistant; Food-borne bacteria; ready to eat Chicken Meat.
ABSTRACT One hundred fifty samples were read to eat chicken meaty collected from different restaurants in Luxor city to evaluate bacteriological quality RTE chicken meat. The mean values of APC, Coliform, Staphylococcus counts, Anaerobic count, Yeast count, Mould count (cfu /g). 1.36X104?2.94X103&8.42x102?3.90x102,4.67?1.15&1.00?0.73&7.67?2.23,2.67?1.26 to chicken shawirma without addition, 5.58X103?1.16X103&3.34x102?9.82x10&2.33 ?1.24&1.00?0.75&8.33?2.40&2.67?1.26 for chicken burger without addition and 4.62X103?7.42X102&1.53x102?4.85x10&1.33?0.79&3.00?1.37&4.33?1.64,1.67?0.84 for fried chicken, all samples not detected for Shigella, respectively.9 E.coli (6%) were isolated from samples represented 4(8%),(2)O125:H2,(1)O143:H8and (1)O111:H1from chicken shawirma without addition, 3(6%) O55: H2, O158:H3and O86a:H1 from chicken burger without addition, 2 (4%) O142:H3, O26:H1from fried chicken, Also, 8(5.33%) Coagulase positive Staphylococcus aureus were isolated from samples. Represented as 4(8%) isolate from chicken shawirma without addition, 3 (6%) from chicken burger without addition, 1(2%) from fried chicken. 6(4%) of Salmonella were isolated from Samples represented as 2(4%) S. typhimurium and 1(2%) S. enteritidis from shawirma without addition, 1(2%)S. enteritidis and 1(2%) S. Anatum from chicken burger without addition, 1(2%) S.Kentucky from fried chicken. Isolates. Most of E.coli isolate sensitive for Colistin sulphate(10ug) and Nalidixic acid(10ug). St.aureus isolates sensitive to Ampicillin (10ug) and Vancomycin (15ug). Most of Salmonella isolate sensitive for Gentamycin (10ug), Colistin Sulphate(10ug) and Ceftriaxone.
EFFECT OF HONEYBEE VENOM (APIS MELLIFERA) ON RESPIRATORY FUNCTIONS OF HYPERCHOLESTEROLEMIC MALE ALBINO RATS / تأثير سم عسل النحل (ابيس ميليفرا) على الوظائف التنفسية لذكور الجرذان البيضاء عالية كوليستيرول الدم
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 11
اسماء رفعت احمد ابراهيم
عزة محمد عبد الرحمن مرعى( azza.marei@fsc.bu.edu.eg - ) - مؤلف رئيسي
ABSTRACT Hypercholesterolemia can be defined as the presence of high plasma cholesterol levels, with normal plasma triglycerides, as a consequence of the rise of cholesterol and apolipoprotein B (apoB)-rich lipoproteins, called low-density lipoprotein (LDL) and characterized by very high levels on the blood cholesterol which is a risk factor of cardiovascular health. Medicines which formed from chemical compounds cause serious side effects that cause an imbalance in the body’s functions. Therefore, using of natural substances may be an innovative method for the treatment of diseases, as they do not have any side effects on human health. Bee venom can be used as a drug due to its beneficial effects on many disorders, such as neurological, cardiovascular, hematological, musculoskeletal, and dermatological disorders. In the present work, we found that treatment of hypercholesterolemic rats with bee venom (BV) revealed significant increases in Hb, Hct, and RBCs count and showed a significant increase in PaO2, % O2 saturation, O2 carrying capacity. These results suggest that BV can enhance respiratory functions of blood and can be used in respiratory diseases to enhance the respiration and hematological parameters. Also, BV could be used as a prophylactic agent (food protector) to protect us from the harmful effects of cholesterol.
EFFECT OF ELECTRICAL STUNNING ON QUALITY OF BROILER CARCASSES / تاثير التدويخ الكهربائى على جودة ذبائح بداري التسمين
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 10
اسماء رفعت احمد ابراهيم
صالح شفيق محمد - مؤلف رئيسي
ايمان شكري جاد( emanshukry81@gmail.com - )
زغلول احمد عبد المنعم خضر
Keywords: Electric – stunning - broiler
ABSTRACT This study aimed to evaluate the effect of electrical stunning on quality of broiler carcasses. Two hundred of apparently healthy broiler Ross 208, 2kg weight, 37 old day from the same flock were divided into two groups, each group of 100 broiler; the first on slaughtered without stunning while the second one was stunned in water bath stunner at 40 voltage for 10 seconds voltage. The results of this study showed that the sensory evaluation including color, texture, appearance, odor and general acceptance were7, 6, 5, 7, and 6 in non- stunned carcasses while in stunned one they were 8,8,7,7, and 7,5, respectively. The mean total bacterial counts were 4.3x105 ±1.07x105 in non stunned carcasses and 3.30 x105 ±1.01 x 105 in stunned carcasses. The good effect of electrical stunning were occurred in carcasses quality because of grade B carcasses were decreased from 15% in non- stunned to 8%in stunned carcasses due to decrease of defects of brocken bone, red wing, wing bleeding spots, from 5%, 4% and, 4% in non stunned carcasses to 1%, 0% and 1% in stunned on although increase of breast bleeding spots from 2% in no stunned carcasses to 6% in stunned one. There is no detectable effect for low voltage water bath electrical stunning on chemical composition of meat (moisture, fat, protein and ash) with increase of pH. In-addition to non-significant increase in anti-oxidants enzymes of slaughtered carcasses. from these study results, we recommend using the electrical stunning before broiler slaughter as it improves the carcasses quality through decreasing the manufacture defects as broken boned and not affects the chemical composition of carcasses meat.
SURVEY OF NEWLY BORN CALVES MANAGEMENT PRACTICE IN NEW VALLEY GOVERNORATE / دراسة استقصائية (استبيان) للممارسات الإدارية للعجول حديثى الولادة في محافظه الوادي الجديد
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 8
اسماء رفعت احمد ابراهيم
نورا عبد الباعث سيد( norabaeth@gmail.com - ) - مؤلف رئيسي
معتز احمد محمد
سطوحى احمد سطوحى
رمضان دردير محمد
Keywords: Newly born calve management, weaning method, calf rearing.
ABSTRACT
The objective of this work was to conduct a broad survey of newly born calves management practices that have an effect on animal behavior and mortality. Animal’s farms were visited during the summer of 2019 in new valley government. Data were collected on 70 farms to answer the questions about Calving pen presence, calves raising method, colostrum feeding number or volume, mortality rate, care of newly born animal, weaning age and method used. The obtained data showed that, 85% of Calves were raised by the owner till fatting and 13 % were pursued from the market, A total of 95% of owners haven’t calving pen in thier frame beside that, 73% of them were fed all colostrum during 6 hours after parturition and 60% of them were feed colostrum two days. On the other hand, 67% of owners did not used disinfectant after parturition which associated with 22% mortality rate of newly born calves. Finally data obtained show that, about 75% of owners were weaned the animal more than 4 month with gradually weaning type.
EPIDEMIOLOGICAL AND MOLECULAR STUDIES ON RIEMERELLA ANATIPESTIFER INFECTION IN DUCKS / دراسات وبائيه وجزيئيه على الاصابه بميكروب رايميريللا اناتيبيستيفيرفى البط
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 14
اسماء رفعت احمد ابراهيم
ضحى عبد الرحمن احمد( dohabdalrahman780@gmail.com - ) - مؤلف رئيسي
ABSTRACT Infectious serositis is a considerable economic problem in duck industry caused by Riemerella anatipestifer. The current study was conducted to investigate the circulating R. anatipestifer in ducks in Assiut Province and assessing their antimicrobial susceptibility. One-hundred and twenty diseased or freshly dead ducks aging 1-18 weeks were examined. Naturally infected birds showed respiratory, nervous, and locomotor disturbances, and low body weight. R. anatipestifer was detected in 16.6% (20) of birds. Among the bacteriologically positive 20 birds, only 10 could be identified by PCR as R. anatipestifer with a prevalence rate of 8.33%. The sensitivity biogram revealed that all the obtained isolates were sensitive to amoxicillin, doxycycline, and flumequine while resistance to streptomycin, chloramphenicol, ampicillin, erythromycin, spectinomycin, and cephradine was observed. On the basis of MIC, all isolates had 90- 100% sensitivity to doxycycline and amoxicillin, respectively. Experimentally, the isolated R.anatipestifer strains showed pathogenicity to 14-days-old ducklings.
DETECTION OF BULLS PERSISTENTLY INFECTED WITH BOVINE VIRAL DIARRHEA VIRUS IN ASWAN PROVINCE, EGYPT / التعرف على الثيران دائمة الاصابه بفيروس الاسهال الفيروسى البقرى فى محافظة اسوان ، مصر
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 12
اسماء رفعت احمد ابراهيم
أمل مختار محمد عبدالعال( amlmokhtar2011@hotmail.com - ) - مؤلف رئيسي
ABSTRACT This study was designed to detect the persistent infection with bovine viral diarrhea virus (PI- BVDV) among 114 bulls in Aswan Governorate. Sera samples were examined by ELISA and real time polymerase chain reaction (PCR) to detect BVDV antibodies and BVDV nucleic acid, respectively. The percent of infection was 23.7% (27/ 114) and 76.3% (87/ 114) were negative by ELISA technique, and 3.4% (3/ 87) for BVDV gene detection by real time PCR. Sensitivity of 89%and specificity of 96 % was reported using the commercially available ELISA kit for samples obtained from PI animals.
ABSTRACT Poultry is an important source of animal protein, as it is considered an essential and effective pillar in filling an important part of the human nutritional needs. The poultry industry has made great progress in recent years and the productivity of poultry has increased significantly with high efficiency as a result of the progress and great efforts that have been made in applied research in various fields science of this industry. During recent years, attention has been paid to using plants with medicinal efficacy as alternates to antibiotics and growth improvement, mostly in the European Union, where antibiotics were banned in the diets of poultry flocks since 2006 due to concerns about increasing microbial resistance to the antibiotics used in poultry diets. The high costs and the possibility of developing microbial resistance to antibiotics has led to an urgent need to use another replacement to antibiotics in poultry feeding, such as the use of probiotics, organic acids, essential oils and phytogenic compounds with medicinal properties for the purpose of maintaining the health of poultry and obtaining the highest production in poultry flocks. The most common feed replacement used in poultry ration are antioxidants, antibacterial materials, enzymes, growth promoters and immune modulators, metabolites or substances that improve the pH and the internal environment of the intestine. Therefore, in this article, we will shed light on the most remarkable feed supplements used in poultry diets for the purpose of improving and increasing production efficiency in poultry field.
SEROLOGICAL DIAGNOSIS OF BOVINE CORONAVIRUS IN CALVES USING A DEVELOPMENT NATIVE ELISA PREPARATION / التشخيص السيرولوجى لفيروس الكورونا البقري في العجول باستخدام إليزا مطورة محليا
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 12/04/2021
تاريخ قبول البحث 24/06/2021
عدد صفحات البحث 10
اسماء رفعت احمد ابراهيم
رانيا صلاح عبد الفتاح المحمدي( rania.elmohamdy@arc.sci.eg - ) - مؤلف رئيسي
ABSTRACT Bovine coronavirus (BCoV) infection has found worldwide among cattle of all ages, causing diarrhea in newborn calves, winter dysentery in adult cattle and respiratory tract diseases results in major economic losses in dairy herds. The objective of this study was to establish an ELISA kits as patent prepared in Lab. with assessment its applicability in the field samples. Sixty-five pneumonic calves 2- to 4-month-old, used in this study to detect BCoV antibodies in serum of infected calves, where it assayed against BCoV antibodies by using prepared ELISA plates and SNT. The results were positive for BCoV antibody 72.3% and 53.9% by ELISA and SNT respectively. The prepared ELISA showed high sensitivity of over SNT, where sensitivity, specificity and correlation were 91.4%, 50% and 72.3% respectively, and there is a positive correlation between both tests. The test has been successfully used in longitudinal field studies on virus dynamics and evaluation of management routines. In conclusion the patent prepared ELISA is more sensitive, accurate and specific assay than SNT for detection of BCoV antibodies, in addition to saving time and commercial kits cost.
MEASUREMENT OF TRANS FATTY ACIDS IN READY TO EAT CHICKEN MEAT / قياس الدهون المتحولة فى لحوم الدواجن الجاهزة للاستهلاك
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 22/06/2021
تاريخ قبول البحث 23/06/2021
عدد صفحات البحث 7
اسماء رفعت احمد ابراهيم( zeinabrenad@gmail.com - )
زينب احمد محمد( zeinabrenad@gmail.com - ) - مؤلف رئيسي
سوزان فوزى السيسى( zeinabrenad@gmail.com - )
Trans fatty acid; ready to eat; chicken meat
High intake of TFA leads to more health problems as heart disease, breast cancer and diabetes. So we worked in this study for measurement of trans fatty acids (TFA) of ninety ready to eat chicken meat samples from restaurants of Luxor city, and effect of different material of fried on TFA to help people and tourists for determined awareness of TFA in these restaurants and material of fried. In this study found mean of TFA and elaidic acid in chicken burger samples (1.813 mg\100 gm, ± 0.151 mg\100 gm, and 1.450 mg\100 gm, respectively). In fried chicken samples (0.159 mg\100 gm, ± 0.024 mg\100 gm, and 0.127 mg\100 gm, respectively), and in chicken shawirma samples (4.016 mg \100 gm, ± 0.583 mg\100 gm, and 3.212 mg\100 gm, respectively). In this study also an experiment to what happens of TFA in ready to eat chicken meat when used of vegetable oil, butter, or margarine was carried out. It was found TFA of chicken burger, fried chicken and chicken shawirma with use of oil (0.343 mg\100 gm, 0.065 mg\100 gm, and 1.622 mg\100 gm, respectively). TFA of chicken burger, fried chicken and chicken shawirma use of butter (1.836 mg\100 gm, 12.855, and 0.446 mg\100 gm, respectively). TFA of chicken burger, fried chicken, Chicken shawirma which use of margarine (3.278 mg\100 gm, 4.139 mg\100 gm, and 3.22 mg\100 gm, respectively). In conclusion: throughout the samples analysis in our study, chicken shawirma samples from restaurants showed the highest content of trans fatty acids and elaidic acid. The TFA formation was found to be varied with the food ingredient and with the material used for frying.
IMPACT OF DIETARY MANGANESE OXIDE NANOPARTICLES ON BIOCHEMICAL PARAMETERS OF CLARIAS GARIEPINUS / أثر تغذية أكسيد المنجنيز متناهي الصغر على الخواص البيوكيميائية في سمك القرموط
قطاع الدراسات البيطرية / General
تخصص البحث : General
Assiut veterinary medical journal / المجلد 67 العدد 168 - يناير 2021
تاريخ تقديم البحث 22/06/2021
تاريخ قبول البحث 24/06/2021
عدد صفحات البحث 9
اسماء رفعت احمد ابراهيم
محمد عبد العزيز أحمد عبد الجليل
أحمد السيد عصمان
نجوى سيد أحمد
محمد عبدالله موسى
ساره رشدى ثابت( sararoshedy0@gmail.com - ) - مؤلف رئيسي
DIETARY MANGANESE OXIDE NANOPARTICLES
This study was conducted on 150 (one hundred and fifty) Clarias gariepinus to investigate the blood lipids (triglycerides and cholesterol), kidney functions (creatinine and urea), liver functions (ALT – AST - ALP - LDH), antioxidants (CAT – SOD - GPx) and growth performance (body weight and weight gain). The study was designed by dividing the fish into 3 groups: group 1: (Control Group), group 2: (2.4 mg manganese oxide nanoparticles/kg ration) and group 3 (1.2 mg manganese oxide nanoparticles/kg ration). Samples were collected on day 15, 30, 45 and 60 of the experiment and blood samples were collected for serum separation. The results of this study indicated that the 2nd group exhibited a significant and progressive decrease in triglycerides, cholesterol, ALT, AST, ALP, LDH, creatinine and urea at all experiment times compared to the control group. Shifting to the antioxidants, the 2nd and 3rd groups showed a significant increase in CAT, SOD and GPx levels in the serum compared with the control group at day 60 of the experiment. Concerning the results of growth performance, both groups reflected a significant improvement in body weight and weight gain compared with the control group.
جميع البيانات التي يتم تسجيلها على النظام هي ملك للجهة صاحبة هذه البيانات
لا يتم الاطلاع على هذه البيانات من قبل مهندسي المركز إلا للضرورة القصوى ولغرض الدعم الفني وبالاتفاق مع الجهة صاحبة البيانات شفوياً أو كتابياً
لا يتم إعطاء أي بيان من بيانات النظام لأي فرد او جهة فرعية تنتمي للجهة صاحبة البيانات او لأي جهة أخرى إلا بموافقة كتابية من الجهة صاحبة البيانات
يمكن استخدام البيانات المسجلة على النظام بهدف عمل إحصائيات يستفاد منها فى قياس اداء النظام وكفاءة استخدامه من قبل مستخدميه
يتم تأمين جميع البيانات الخاصة بالنظام من خلال تأمين الحاسب الخادم الذي يستضيف البيانات ومن خلال تأمين انتقال البيانات بين جهاز المستخدم والحاسب الخادم وكذلك تأمين واجهة النظام التي يستخدمها المستخدم
كل مستخدم للنظام مسئول مسئولية كاملة عن كلمة المرور الخاصة به وعليه اتخاذ كافة الاحتياطات الممكنة حتى لا يستطيع أى شخص آخر الحصول عليها
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