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[9003507.] رقم البحث : 9003507 - |
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ENCAPSULATION OF ANTENNAPEDIA (PENETRATIN) PEPTIDE IN A POLYMERIC PLATFORM FOR EFFECTIVE TREATMENT OF INTRACELLULAR BACTERIA / تغليف الببتيد انتينابيديا (بينتراتين) في قالب بوليمري لعلاج فعال للبكتيريا داخل الخلايا |
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قطاع الدراسات الصيدلية / الصيدلة الصناعية |
| تخصص البحث : الصيدلة الصناعية |
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Bulletin of Pharmaceutical sciences / |
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سارة يوسف محمد ( Sarahyousef30@yahoo.com - ) |
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د/ مروة جلال النجار - مؤلف رئيسي |
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الصيدلة الصناعية |
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Antimicrobial peptides (AMP) and cell-penetrating peptides (CPP) are two classes of peptides that share some structural and physicochemical similarities. Antennapedia or penetratin (ANT) is one of the most known CPPs, that was proven to have antimicrobial activity against certain strains of planktonic bacteria. ANT can enter the cells but has no activity against intracellular bacteria. This is attributable to the inability of the peptide to reach bacteria reside within cellular components as well as low delivery efficiency, due to loss of activity by proteolysis and poor specificity. The aim of this work is to develop a formulation that can effectively reach and attack intracellular bacteria. To achieve this goal, ANT was encapsulated in PLGA platform as nanoparticles with the size range of 500-1000 nm, which allows for selective uptake by macrophages where bacteria mostly reside. ANT was loaded with high loading efficiency (12.7%) inspite of high water solubility. ANT-nanoparticles (ANT-NP) had no cytotoxicity on J774a.1 macrophages and were readily taken up by macrophages as confirmed by fluorescence microscopy. Antibacterial activity of ANT-NP remains to be tested against different intracellular bacteria. |
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