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[9004685.] رقم البحث : 9004685 - |
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Identification of Sex-specific Molecular Markers in Barbel and Nile Carp using SCoT and ISSR Markers / |
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قطاع الدراسات الزراعية / General |
| تخصص البحث : General |
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Assiut Journal of Agricultural Sciences / |
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هناء مصطفي اسماعيل عثمان ( 11 ) |
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آية أحمد محجوب - مؤلف رئيسي |
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السيد عبد المنصف محمد |
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محمد أحمد الملقب بالخرشي |
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وجمال إبراهيم أحمد محمد |
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SCoT, ISSR, Cyprinidae, sex differentiation |
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The present study aimed to investigate molecular sex differences between males and females of two members of family Cyprinidae in Egypt namely Benni and Lebeis using two different molecular markers i.e., start codon tar-geted polymorphism (SCoT) and inter simple sequence repeats (ISSR). The bulked DNA sample for each gender of the tested species was screened with eight primers from each marker. However, SCoT marker was more efficient than ISSR marker by showing higher number of possible sex-specific bands in the two tested species. SCoT primers were able to generate 7 and 4 male-specific bands, along with two and 5 female-specific bands for B. bynni and L. niloticus, respectively. |
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