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[9000484.] رقم البحث : 9000484 - |
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In vito Propagation of Salvadora presica l., Compartive Phytochemical Screening and Antimicrobial Activity of The Propagated and Wild Plant. / |
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Agricultural Research Journal Suez Canal University / |
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mohamed abdel fattah mohamoud |
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helmy belal |
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mohamed mohei el din |
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magdy gonehim |
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alaa mohamed mohamed |
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antimicrtobial activity. wild plant. |
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The growth response of S. persica in vitro via micropropagation using nodal cutting explants was investigated. The effect of kinetin, indole acetic acid and type of nutrient medium on the growth of nodal cutting cultured in vitro after 8 weeks in starting stage was studied. The influence of kinetin, indole acetic acid treatments and their combination on the Murashige and Skoog (MS) basal medium full strength was determined in the multiplication stage. The highest rooting percentage was obtained on MS medium supplemented with 2.0 mg/l Indole acetic acid without addition of active charcoal. Preliminary phytochemical screening identifies the presence of carbohydrates and/or glycosides, free flavonoids, sterols and/or triterpenes, alkaloids as well as tannins more strongly in the shoot samples propagated from tissue culture technique indicating their occurrence in such samples with higher amounts. The UV and the visible spectra were recorded by the spectrophotometer for the tested extracts prepared from both the wild and tissue culture propagated plant of S. persica. The UV/visible spectra of the ethanol 95% extracts of both the wild and tissue culture propagated plant were similar. The intensities of the absorption bands were higher with the extracts prepared from the plant propagated by tissue culture technique. The extracts of S. persica propagated by tissue culture technique exhibited a broad spectrum antimicrobial activity against the tested microorganisms, meanwhile the extracts of the wild plant showed less activity. |
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