الفهرس 
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Abstract
In the present study the genotypic identification of both virulence and antimicrobial resistance factors indicate an Association between virulence and antimicrobial resistance in S. aureus and S. agalactiae, sample included 522 dairy cows were examined during the period from July 2019 to August 2022 in El-Sharkia governorate.
The study showed that the prevalence of mastitis at the cow level was (31.22%), (13.79%) for clinical mastitis and (17.43%) in case of subclinical mastitis, respectively. The prevalence at the quarter level was (18.01%), out of them (7.14%) in case of clinical mastitis and (10.96%) in case of subclinical mastitis. Bacterial culture revealed that predominant isolated bacteria was Staphylococcus aureus (48.27%) followed by coagulase negative staphylococci (29.09%) and Streptococcus spp. (22.63%). (nuc) gene was used to confirm (25) isolates of S.aureus. Among them only two isolates (8.00%) revealed methicillin resistant (positive mecA). Seventeen isolate were CoNS with five isolates (29.41%) among them were methicillin resistant (positive mecA). By using Conventional PCR assay targeting (Tuf) gene 16S rRNA (Streococcus genus specific) was used to confirm 17 Streptococcus isolates , eight S.agalactia and two S.ubris isolated were confirmed.