الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 99 |  |  |
| | | from | 99 | | |
Abstract
The aim of this study was to evaluate the role of Co-oxide@gold core shell nanoparticles in
the diagnosis of breast cancer. Also, this proposal aims to explore the photothermal effect of Co-
oxide@gold nanoparticles in treatment of breast cancer.
The experiment was carried out on a total of 60 female Swiss albino mice injected
intramuscularly by 2*106 Ehrlich ascites carcinoma cells, diluted approximately (1-4) in 0.9
% saline in the breast. To investigate the efficiency of gold nanoparticles as contrast agent for
X-ray imaging and cancer treatment.
For evaluation of Co-oxide@gold nanoparticles as x ray contrast agent, when tumor
reaches approximately 1-5 mm in diameter, mice were randomly divided into two main Group
I: tumor of animals of this group were visualized with x ray imaging; group II: tumor of
animals of this group were visualized with x ray imaging after injecting Co-oxide@gold
nanoparticles via tail vien injection.
For evaluation of the hyperthermal effect of gold nanoaprticles; when tumor reaches
approximately 1-5 mm in diameter, mice were randomly divided into the following groups,
each of ten mice as follows:
group 1: Tumor bearing mice injected with phosphate buffer saline as control
group.
group 2: Tumor bearing mice injected injected with gold nanoparticles only via tail
vein injection.
group 3: Tumor bearing mice injected with phosphate buffer saline and irradiated
with laser beam irradiation.
group 4: Tumor bearing mice injected with gold nanoparticles via tail vein and
irradiated with laser beam irradiation.
The laser exposure was 10 minutes on each time day by day during one week. To study
the hyperthermal effect of laser exposure, the following parameters were estimated: tumor
volume (mm3), tumor volume ratio (TVR), inhibition ratio of tumor, mice survival time and
life span and determination of total antioxidant (TAC) and malondialdehyde (MDA). In
addition to histopathology of the tumor to study treatment effect.Animal weight, white blood
cell counts and liver enzymes activity were assayed as general markers of toxicity as well as
the histopathology of the liver and general toxicity signs (loss and physical activity).
Our results can be summarized as follow:
- As x ray contrast agent, Co-oxide@gold core shell nanoparticles and gold nanoparticles did not
show any ability to image tumor mass in breast tissues of female mice; while combination of
gold nanoparticles and silica nanoparticles shower higher ability as contrast agent to visualize
tumor mass in breast tissues of female mice.
Summary, Conclusion & Recommendation
65
- Animals injected with gold nanoparticles prior to laser irradiation showed significant
reduction in tumor volume compared with animals irradiated with laser only after 3 and 7
days.
- The mean tumor volume of animals injected with gold nanoparticles prior to laser
irradiation after 7 days was 1.41 times greater than that after 24 hours post irradiation with
tumor inhibition ratio of 72.1 compared with control group.
- The mean survival time of mice injected intratumoral with GNPs and irradiated with laser
beam were significantly higher than that of control group, while no other groups showed
significant difference.
- Animals injected with GNPs prior to laser irradiation (three times) showed non-significant
difference in concentration of MDA after 1, 3 and 7 days post irradiation compared with
control group, but was significantly lower at 3 and 7 days post irradiation compared with its
corresponding group that did not injected with GNPs (GpIII).
- Animals injected with GNPs prior to laser irradiation (three times) showed non-significant
difference in TAC levels after 1, 3 and 7 days post irradiation compared with control
group, but was significantly higher at 3 and 7 days post irradiation compared with its
corresponding group that did not injected with GNPs (GpIII).
- All animals were free of toxic clinical signs throughout the experiment period of 7 days.
Mice injected with GNPs prior to laser irradiation did not cause body weight changes
compared with the control group during 7 days post irradition.
- Animals injected with GNPs prior to irradiation with laser (three times) showed significant
reduction in WBCs count compared with control group but higher than their corresponding
groups that did not injected with GNPs (GpIII).