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Abstract
An experimental was conducted to estimate the contamination of plant, soil and water after plant treatment using the recommended pesticides at the recommended dosages for pest control.
Tow popular vegetable crops were used in this study i.e. green bean (Phaseolus vulgarus) and green peas (Pisum sativum). Green bean and seeds were planted at menofia governorate on February 26th, 2005 inplots 0.01of feddan each. Every 3-randomized plots were treated on may 11th, 2005 with one the following pesticides.
1: fenitrothion 50% EC formulation at the rate of 1 L/feddan.
2: Etoxazole 10% SC formulation at the rate of 40 ml/feddan.
A knapsack sprays, was used for appluing the previous doses after it had been diluted with 200liters of water per feddan.
Chemical study of pesticides residues.
A- In plant tissues
Representive leaves for each replicate were picked at random one hour after treatment. The following samples were taken 1, 3, 6, 9, 12, 15 and 21 days after application. Representative samples of green bean pods and green peas seeds were taken at the same previous intervals. All samples were kept in polythylene bags at – 20C0 in deep freezer.
Samples were processed and cleaned up. Residues of fenitrothion were subjected to GC analysis, while residues of etoxazole were subjected to HPLC analysis. Residues of the previous pesticides were subjected to GC-MS analysis.
B- In soil
Soil samples were taken randomly from the upper 0-10cm layer of the treated plots. Initial samples were taken one hour after application. Subsequent samples were taken 1, 3, 6, 12, 19, and 35 days after treatment. Then samples were kept and stored at -20C0 in deep freezer until time of analysis.
Samples were extracted then fenitrothion and etoxazole residues were subjected to the analysis previously used for plant samples.