الفهرس | Only 14 pages are availabe for public view |
Abstract This study try to create genetic variations in pea variety Master B to induce better characteristics regarding nodulation and nitrogen fixation process using gamma irradiation furthermore, perform and produce conjugation between Pseudomonas putida (donor) with four different rhizobial strains (reipents) and Azorhizobium microsymbiont as a mechanism of DNA transfer. This would facilitate releasing a variety of recombinant rhizobia into the environment this with improving nitrogen fixation capacity. In conclusion, it is evident that it is possible to select rhizobial strains harboring recombinant genomes that were efficient in secrete IAA as a plant hormone, as well as, salicylic acid as a inhibitor agent against pathogenic microorganisms. Bacteriocins produced from these recombinant strains had a good performance to inhibit the growth of Agr1513 as an soil pathogen. In addition, rhizobial recombinants appeared symbiotic effectiveness leading to increase grain yield, as well as, improving biochemical traits such as grain protein contents and leaves chlorophyll concentration. |