الفهرس 
Aim of the work
Basal cellsOnly 14 pages are availabe for public view
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Abstract
Background: Recently, a spontaneous transgenic mouse model of prostate cancer, TRAMP, was developed (Greenberg et al., 1995). Methods: Nine cell lines Primary and Metastasis cells were established from a heterogeneous (3241week) tumor of the transgenic line of C57Bl/6 mice. Cytokeratin, Synaptophysin, T antigen oncoprotein, Vimentin, and p53 were estimated by immunohistochemistry. Fas expression, Ecadherin, CD40 and CD44 were estimated by flow cytometry. Apoptosis was estimated by CalceinAm assay, thymidine uptake and Internucleosomal DNA cleavage. Results: Cells had similar doubling time at 24 h. Cell types estimated by DiffQuick . Cells were cloned and one clone from every cell line was isolated. Cytokeratin and Synaptophysin are not expressed in all cell clones; the T antigen oncoprotein is expressed in all cell clones. Seven cell clones are expressed vimentin, p53 expressed in six cell clones. Ecadherin did not express in two cell clones and but express at low level in other seven cell clones. CD44 expression was variable in the cell clones. The mice did not develop any tumor when injected S.C. to intact C57Bl/6 male mice Three cell clones were screening for androgen dependency and these were found to be androgenindependent. Nine cell clones were found to be reactive with antiFas mAb. TRAMPC2 and one cell clone were resistant; five cell clones were slightly sensitive but two cell clones were sensitive to Fasmediated killing. It was found that one cell line only inhibited the cell proliferation using a thymidine uptake assay. Internucleosomal DNA cleavage was observed after incubation with 1mg antiFas for 24h in TRAMP C2 and six cell clones. CHX itself induced apoptosis in TRAMPC2 and nine cell clones. CHX did not affect Fas sensitivity of TRAMPC2 and four cell clones, but increased Fasmediated apoptosis in the other five cell clones. Internucleosomal DNA cleavage was observed after incubation for 24h with CHX alone in TRAMPC2 and three cell clones (25mg CHX), three cell clones (0.25mg, 2.5mg, 25mg CHX), one cell clone (2.5mg, 25mg CHX), but after incubation with CHX in presence of 1mg aFas, we found Internucleosomal DNA cleavage in TRAMPC2 and six cell clones (0.25mg, 2.5mg, 25mgCHX), three cell clones (2.5mg, 25mg). CD40 were negative in all cell clones. HU did not show significant effect either with HU alone or in the presence of antiFas in PC3mock. It did not shown any cell killing in one cell clone with HU alone or in the presence of antiFas. Treatment of three cell clones with HU increased the effect of Fas by using CalceinAm assay. Internucleosomal DNA cleavage was observed in one cell clone after incubated cells with HU either alone or in the presence of antiFas for 24h, but in one cell clone, weak DNA ladder after incubated cells with HU either alone or in the presence of antiFas for 48h was observed