الفهرس | Only 14 pages are availabe for public view |
Abstract The aim of this work was to study the effect of different histo¬!!thological lesions observed, in ’kidney transplant biopsies on the hort and long term graft outcome. Two hundred and five kidney transplant biopsies taken from 161 (atients were critically evaluated. Renal biopsies were formaline fixed and paraffin sections were tained by haematoxylin and eosin, periodic acid schiff, Masson rtchrome, periodic acid silver methenamine and congo red stains. In . orne cases phosphotunges ti c ad d haematoxyl in stai n was used to ernonstrate areas of fibrinoid necrosis. In 25 cases a second core was taken for frozen sections to be eva¬uated by direct immunofluorescence, and stained by fluoresceine con¬ugated antihuman IgG, IgA, IgM, C3, C1q’ C4 and fibrinogen, and xarnined by immunofluorescent microscopy. Different histologic lesions were evaluated in each biopsy and cored according to its severity. The lesions evaluated in our cases ’ere: mesangial matrix thickening, glomerular capillary thrombosis, lomerulitis, prescence of crescents, glomerulonephritis, immune depo¬its, tubular necrosis, tubular infilteration by inflammatory cells ubular atrophy, interstitial infilteration, interstitial oedema, .nterstitial hemorrhage, interstitial fibrosis, endovasculitis, fibri¬(oid necrosis, arterial thrombosis, intimal fibrosis and infarction. |