الفهرس | Only 14 pages are availabe for public view |
Abstract The current study aimed to isolate and diagnose four types of multi-antibiotic-resistant bacteria that infect diabetic foot patients, study the effect of some essential oils on them, and evaluate their effect on the phenotypic appearance of those bacterial isolates taken from diabetic foot patients, 200 samples were collected from both sexes. A swab from diabetic foot patients from Mansoura university Hospital, and the samples were brought to the laboratories of the Microbiology Diagnostic and Infection Control Unit (MDICU) in the Department of Medical Microbiology and Immunology, of the Faculty of Medicine at Mansoura University, and the samples were isolated to identify the bacteria, which are Escherichia coli, Staphylococcus aureus, Proteus mirabilis, and Pseudomonas spp. and were grown in Petri dishes and on special media for each type for the purpose Bacteria were isolated and diagnosed, and a sensitivity test to 14 antibiotics was performed. The most resistant isolates were selected and grown again for the purpose of treating them with plant extracts of essential oils for each of (green tea, cloves, garlic, Allium sativum, and Nigella sativa), and then scanning was performed. Electron microscopy for TEM preparation and Transmission examination to determine the extent of the effect of clove extract on each of the four bacterial species and to compare control isolates with isolates treated with it. The current study concluded that cloves are the most effective plant extract on multi-antibiotic resistant bacterial isolates that affect diabetic foot patients. The results of the statistical analysis recorded significant differences when comparing the culture media of the control isolates to the media treated with cloves, The results of the electron microscope images recorded changes in the morphology of the bacterial cell of the four bacterial genera in the study. The isolates treated with clove extract were clearly different from those not treated with any type of plant extract. This proves the effectiveness of cloves as an antibiotic for multiantibiotic-resistant bacteria. The current study recommends conducting additional studies on different plant extracts. In general, and cloves in particular for use as antibacterial agents for multi-antibiotic resistant bacteria |