الفهرس 
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Abstract
The present study provides much evidence of the anti-oxidative, neuroprotective and anti-inflammatory effects of green tea and pumpkin oils against inflammation in the hippocampus of MOG- induced male albino rats.
In this study, the adult male albino rats were divided into four groups, six for each were designed as follow:
group I (Normal control group): Animals were given an equivalent volume of saline (0.9% NaCl) intraperitoneally for 2 weeks (one time/week), in addition to an equivalent volume of 1% carboxymethylcellulose (CMC) (1 % w/v) orally as vehicle every other day alternatively till the end of the experiment (17 weeks). Starting from the 3rd week, rats were given an equivalent volume of 1% tween 80, orally four times per week for 3 weeks.
group II (control group administrated with MOG): represents an experimentally induced MS group in which the MOG-administrated is used. The animals will be immunized by the injection of the peptide subcutaneously. On days, 0 and 2, pertussis toxin is injected intra-peritoneal relative to immunization.
group III (MOG administrated group treated with Green tea oil): In the MOG-administrated group treated with GTO, the rats were gavaged daily with 5 ml of GTO per kilogram of body weight (Ganji et al., 2019). The treatment was started at the onset of clinical symptoms and was continued for 21 days. Clinical symptoms of the disease initiated between days 12 and 19 post-immunization.
group IV (MOG administrated group treated with pumpkin oil): In the MOG-administrated group treated with PO, the rats were gavaged daily with 5 ml of PO per kilogram of body weight (Ganji et al., 2019). The treatment was started at the
onset of clinical symptoms and was continued for 21 days. Clinical symptoms of the disease initiated between days 12 and 19 post-immunization.
In the current study, the animals induced by MOG showed significantly decrease of Dopamine, Serotonin, acetylcholine and Norepinephrine activities as well as the elevation of epinephrine levels. The treatment of MOG- induced rats with GTO and PO improve these changes.
The attained data also showed a significant increase in serum LPO and decrease in GSH, SOD, and GPx, Q10, α-tocopherol and NRF2 activities. The treatment of MOG- induced rats with GTO and PO led to marked amendment in these parameters changes.
The administration of MOG produced an elevation in pro-inflammatory cytokines (INOS and IFN-β) activities and P53 levels. As well as decreasing in BCL2 activity. The treatment of MOG- induced rats with GTO and PO improve these alterations.
Regarding to oxidative stress in brain and spleen tissue, the administration of MOG produced an elevation in LPO and decreasing in SOD, GSH, and GPx contents. The treatment of MOG- induced rats with GTO and PO provides significant improvement of these changes.
On the other hand, the brain of MOG-administered rats led to histological change that show infected oligodendroglial cells, neuronal cells, and macrophages. In addition, mononuclear inflammatory cellular infiltrate (arrow) & hemorrhage, a focal area of necrosis plaques (stars) and marked edema. The treatment of these animals with GTO&PO successfully prevented most of these biochemical and histological alterations.
In conclusion, the possible anti-oxidative, neuroprotective and anti-inflammatory effects of green tea and pumpkin oils on MOG-induced rats, mechanisms may be
explained on the basis of oxidant-antioxidant system management, regulation of the inflammatory status as well as modulation of the apoptotic pathway.