الفهرس | Only 14 pages are availabe for public view |
Abstract This study is aiming to evaluate the efficacy of the PDA/n-HA coat in improving the mechanical and biological properties of the 3D PLA scaffold. The fused deposition modeling technique was used to print blank PLA, PLA with embedded n-HA particles, and PLA scaffold coated with PDA/n-HA by immersion. After matrices characterization for their chemical composition and surface properties, testing the compressive strength was pursued using a universal testing machine. The bioactivity of scaffolds was evaluated by monitoring the formation of calcium phosphate crystals after simulated body fluid immersion by Xray diffraction analysis. The compatibility of the scaffolds was evaluated by cell viability (MTT) assay, Also the evaluation of cell adhesion and proliferation was assessed using Scanning electron microscope and cone focal microscopy. This study explored the efficacy of the PDA/n-HA coat in improving the mechanical and biological properties of the 3D PLA scaffold. The bioinspired PDA/n-HA coat did not affect the compressive strength of the PLA scaffold negatively, in contrast to PLA/n-HA scaffold. Moreover, the naturally derived PDA coat incorporated with n-HA has shown excellent biocompatibility with increased calcium phosphate deposition, almost without cytotoxic effects. The enhancement of cellular activity and cellular adhesion shown by the PDA/n-HA coated scaffold serves the purpose of having better bone formation and regeneration for the in vivo translation of the PLA/PDA/n-HA platform. |