الفهرس 
Aim of the WorkOnly 14 pages are availabe for public view
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Abstract
Background and Objective: There is a growing need to comprehend the potential outcomes of nanoparticles on human well-being, including their potential for detecting and treating leukemia. This study examined the role of iron folate core-shell and iron oxide nanoparticles in inducing apoptosis and altering the expression of the Bax, Bcl-2, and Caspase-3 genes in leukemia cells. Methods: The obtained iron oxide and iron-folate core-shell nanoparticles were analyzed using a variety of analytical techniques, including Ultraviolet visible (UV-vis) absorption spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Dynamic Light Scattering (DLS), Zeta potential (ZP), and Transmission electron microscopy (TEM). Additionally, FTIR and UV-Vis were used to characterize doxorubicin. The MTT test investigated the cytotoxicity of iron oxide and iron-folate core-shell nanoparticles. The expression of the apoptotic signaling proteins Bcl-2, Bax, and Caspase-3 was evaluated using the RT-PCR method. Additionally, flow cytometry was performed to gauge the degrees of necrosis and apoptosis.
Results: UV-visible analysis showed that the generated iron oxide and iron folate core-shell NPs had a distinctive absorption curve in the 250–300 nm wavelength range. The XRD peaks were also discovered to index the spherical form with a size of less than 50 nm, which validated the crystal structure. The FTIR analysis determined the bonds and functional groups at wavenumbers between 400 and 4000 cm-1. A viable leukemia treatment approach is a nanocomposite consisting of Iron and an iron-folate core-shell necessary for inhibiting and activating cancer cell death. The nearly resistant apoptosis in the CCRF-CEM cells may have resulted from upregulating Bax and Casepase-3 while downregulating Bcl-2 expression.
Conclusion: Our study documents the successful synthetization and characterization of iron oxide, which has excellent anticancer activities. A metal oxide conjugation with the nanoparticles’ core-shell enhanced the effect against acute leukemia.
Keywords: Leukemia; Iron oxide; Iron-folate core-shell; apoptosis; Bcl-2; Bax; Caspase-3.