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Abstract This chapter includes description of the materials, reagents, equipment and the methods used for the preparation of polymer blends based essentially on proteins with various compositions of some natural polymers such as zein and wheat gluten blending with PVB will be investigated by the solution casting technique, as the applicability of the prepared blends in the form of films blends will depend in the first place on thermal properties, antimicrobial resistance, radiation stability and film forming properties…ect. (I) Preparation of Zein/PVB Film Blends Blend was prepared by Stock solutions containing of (Zein + PVB) with different ratios of PVB to Zein dissolved in ethanol/Isopropanol mixture and the required ratio of glycerol based on blend weight was added to the Summary and conclusion XII solutions. Films were then prepared by solution casting onto Plastic Petri Dishes, and placed on a horizontal-leveled surface. Solutions were allowed to dry overnight in room temperature. (II) Preparation of Wheat gluten/PVB Film Blends Blend was prepared by Stock solutions containing of (WG + PVB) with different ratios of PVB to WG dissolved in Acidic distilled water/Isopropanol mixture and the required ratio of glycerol based on blend weight was added to the solutions. Films were then prepared by solution casting onto Plastic Petri Dishes, and placed on a horizontal-leveled surface. Solutions were allowed to dry about 48h in room temperature. In addition to description of the preparation of scaffolds for the used Tissue Culture (Vero cells & Paulownia tormentors), all aseptic procedures regarding preparation of scaffolds were showed and explained. The different parameters affecting on preparation and the application process are in |