الفهرس 
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Abstract
Acute myocardial infarction (AMI) is a life-threatening disease. It is a main cause of death all over the worldwide.
Early and rapid detection, diagnosis, and early prevention of acute myocardial infarction are demanded to stop the progressive development of AMI.Despite cardiac troponins and CK-MB are preferred biomarkers for myocardial injury, they have their own pitfalls.
Many LncRNAs have highly specific to myocardial tissue. This triggers for the idea of testing its potential as a novel diagnostic biomarkers for AMI.
Therefore, the present study was designed to evaluate the diagnostic performance of lnc FENDRR and Lnc1538 in acute myocardial infarction patients.
The study was conducted on 98 participants who were divided to three groups:
group I: 25 healthy subjects
group II: 23 non-cardiac chest pain patients.
group III: 50 AMI patients.
The specimens collected in this study were blood samples, where the collection procedures were approved by the Research Ethical Committee of Faculty of Medicine, Ain Shams University, Egypt. AMI patients recruited from Ain Shams University Hospital with acute and ongoing chest pain for 8 hours. AMI patients was diagnosed on the basis of elevated serum troponin levels, CK-MB in addition to clinical symptoms and history consistent with recent ECG abnormalities. Non-cardiac patients who were suffering from chest pain but after examination and some analytical tests, they were diagnosed as non-cardiac chest pain.
All participants were subjected to:
- Determination of serum lipid profile
- Determination of troponin I and creatine kinase.
- Determination of lncFENDRR and lnc1538 expression levels.
The results of the study were summarized as follows:
1-There was no significant difference in age, sex, BMI, hypertension and diabetes state among the three groups, while AMI patients showed a significant difference in smoking state when compared to the control group
2- Non-cardiac chest pain and AMI patients showed non-significant difference in levels of total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein-cholesterol. However, levels of triglycerides was increased in AMI patients compared to non-cardiac chest pain and normal controls.
3- Serum CK-MB was significantly elevated in non-cardiac chest pain and AMI patients. In the same context, AMI patients exhibited a significant increase in levels of troponin I when compared to non-cardiac chest pain and normal control group.
4- Lnc FENDRR was significantly downregulated in AMI patients compared to non-cardiac chest pain and normal controls. Whereas, there was no significant difference between non-cardiac chest pain and control in lnc FENDRR expression levels. However, lnc1538 was significantly overexpressed in AMI patients compared to non-cardiac chest pain and healthy control.
5- The ROC curves showed that troponin I had diagnostic value for AMI with 90% sensitivity and 91% specificity. Whereas, lnc1538 had the highest diagnostic value with a sensitivity and specificity of (100% and 98%). While, Lnc FENDRR had diagnostic value with a sensitivity of 93.3% and specificity of 90.5%. So, overall lncFENDRR and lnc1538 showed a potential efficacy to discriminate between AMI patients from those without.
Conclusion
In conclusion, measurement of the expression levels of Lncfendrr and Lnc1538 could be a useful as non-invasive biomarkers for differentiate patients with AMI from control and non-cardiac chest pain patients. The expression levels of Lncfendrr and Lnc1538 can also reflect the stage and severity of AMI.
Recommendations
Further studies will focus on developing a clinically useful early diagnostic test for AMI through evaluation of Lncfendrr and Lnc1538 target genes and will validate the current data in larger sample size study.