الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
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Abstract
SUMMARY
This study was conducted at Micropropagation Technology Laboratory (MPT) at the Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt and in Genetics Department of the Faculty of Agriculture - Ain Shams University - during the period from 2018-2023 on the potato cultivars Diamante and Desiree.
The main target is the isolation of proline-rich protein gene from potato plant under drought stress using Rapid amplification of cDNA ends (RACE) to be used later for enhancing drought resistance.
The explants used in this study were selected from in vitro plantlets of the two cultivars Desiree and Diamante under sterilized conditions in a laminar flow and used as a source of the explants in the subsequent experiment for drought stress treatments by using polyethylene glycol (PEG). Explants from Desiree and Diamante cultivars were planted on Murashige and Skoog (MS) medium with four different concentrations of PEG; C=0mg,T2=2mg,T4=4mg and T8=8mg. Three explants were cultured in each jar and replicated five times for each cultivar.
After six weeks on MS medium supplemented with PEG treatments, five traits were measured; leaf area trait was measured using portable area meter LI-COR machine, lengths of shoot and root trait was measured by c.m, number of branches trait was counted, chlorophyll content trait was measured by Chlorophyll fluorometer CCM-200plus machine and proline content was determined with the method of the colorimetric assay using a UV-visible spectrophotometer (Bio-RAD) at absorbance of 520 nm as mg g −1 Dw.
The obtained results could be summarized as follow:
1- The data were analyzed after six weeks of the PEG-induced drought treatment analyzed by the SAS System, t-tests, Least Significant Difference (LSD).
2- The results of PEG drought stress in a high concentration of PEG (8mg) for leaf area trait values was decreased from 8.06 cm2 to 5.44 cm2 for Desiree cultivar.
3- Lengths of shoot and root trait values were decreased in Desiree cultivar from 29.33 to 27.33 mm rather than the increased value of Diamante cultivar to 30.66 mm compared with control value of 20.50.
4- Total chlorophyll content trait of potato plantlets decreased to 4.16 and 4.56 μg cm-2 for Desiree and Diamante cultivars, respectively, compared with their control.
5- The content of proline trait increaed up to 0.79 µg/ml for Desiree cultivar, while Diamante cultivar showed insignificant difference between control and all treatments.
6- Therefore the proline content trait was the best choice to choose Desiree cultivar for the isolation of proline-rich protien gene.
7- Additionally, The RNA was isolated from Desiree cultivar plants which was treated by a high drought stress (PEG 8 mg) that was transcripted to cDNA to be used in RT-PCR technique.
The RACE technique was used and the PCR gave different fragments as well as the expected fragments after nested PCR that were cloned in a pGEM-T easy vector. The gene was submitted in the Gene Bank under accession number Bank ID: ON922536.