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Abstract
Lumpy skin disease (LSD) is an economically important arthropods porn viral disease of cattle. Lumpy skin disease virus (LSDV) is a Capripoxvirus that belongs to subfamily Chordopoxvirinae of family Poxviridae. LSDV is the etiological agent of LSD. Capripoxvirus members are a group of genetically and antigenically similar viruses. LSD is endemic in most countries of Africa and the Middle East, and is an exotic threat to European countries. Live attenuated SPPV is used as a vaccine in endemic LSD control. Cattle vaccinated with a LSD vaccine containing SPPV seed can develop LSD due to induction of partial protection in vaccinated animals, or as a result of vaccine seed contamination with non-highly-attenuated LSDV. LSDV field control efforts and vaccine production require differentiation between LSDV and SPPV. PCR assays that differentiated LSDV from other capripoxviruses were described; however, the techniques used were either dependent on size differentiation of PCR products, the use of restriction enzymes, or melt-curve analysis. In this study, a simple LSDV-specific PCR assay was developed to reliably differentiate between both viruses. Primers were designed based on whole genome sequence analysis to amplify LSDV DNA spanning parts of an envelope protein gene and an adjacent core virion protein gene.