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العنوان
Preliminary study for detection of capillaria philippinensis copro - DNA in diarrheic patients /
الناشر
Mona Ibrahim Ali ,
المؤلف
Mona Ibrahim Ali
تاريخ النشر
2014
عدد الصفحات
129 P. :
الفهرس
Only 14 pages are availabe for public view

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Abstract

Capillaria philippinensis is a parasite of the small intestine that causes a severe enteropathy and at times death in cases of delayed diagnosis or inappropriate treatment. Molecular - based approaches offer greater sensitivity and specificity over the existing diagnostic tests and it can help in the diagnosis of capillaria infected cases. The aim of our study is to develop a molecular diagnostic method, by utilization of PCR - based assay for detection of C. philippinensis. This study was conducted over 250 patients attending the outpatient clinics of Beni-Suief University hospital. Stool samples were collected and subjected to copro-parasitological examination and copro- nested PCR (nPCR) assays. By microscopic examination, C. philippinensis was detected in stool samples of 36 (24%) individuals, all were complaining of chronic diarrhea