الفهرس | Only 14 pages are availabe for public view |
Abstract This study was carried out during the 2017 and 2018 seasons on mango fruits (Mangifera Indica L.) Naomi and Sensation cultivars. The study was conducted in a private orchard located in Cairo Alexandria desert road. The trees were received the standard horticultural care adapted in the area. The full matured fruits were picked manually by hand, packed in carton boxes and taken directly to the Post-Harvest Laboratory in Horticulture Department, Faculty of Agriculture, Cairo University. The sound fruits were selected. All fruits were washed with regular tap water and soap and then rinsed with water. Fruit uniform in size and good in quality were selected. 225 fruits for each cultivar were chosen, fruits were treated by five treatments of coatings, each treatment consisted of 45 fruits, then were divided into three replicates, 15 fruits for each replicate. This was done for the first and the second season of study. The treatments were as follow Starch, Olive oil, Beeswax, Sodium Benzoate and Control. It was attributed to the fact that the beeswax reduced the evaporation, improved the texture of fruit and hence delayed its wrinkling and softening during the storage period. As the ripening of mango fruits is characterized by loss of firmness due to cell wall digestion by pectinesterase, polygalacturonase and other enzymes. This study aimed to use a technique RAPD and ISSR Based on polymerase chain reaction technology PCR In the analysis of genetic variance between two mango cultivars (Naomi and Sensation), a statement RAPD showed that the percentage of similarity between Naomi and Sensation reached 91.4%, while it was 82.4% when using the ISSR technique. The ISSR and RAPD technology were able to create unique and distinctive DNA packets capable of distinguishing between the studied mango cultivars, That is, these bundles were found in the Naomi cultivar and absent in the Sensation cultivar, and they can be used as a distinctive genetic fingerprint for both cultivars. This study also helped to determine the degree of genetic proximity and distance between them, which contributed to revealing the genetic diversity between the cultivars of Naomi and Sensatio |