الفهرس | Only 14 pages are availabe for public view |
Abstract Diabetes mellitus is defined as a group of metabolic disease characterized by chronic hyperglycemia resulting from defects in insulin secretion, insulin action or both resulting in impaired function in carbohydrate, lipid and protein metabolism. Diabetes cause due to either the pancreas not producing enough insulin, or the cells of the body not responding properly to the insulin produced. It is characterized by chronic hyperglycaemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action, or both. Ginger and Alpha-lipoic Acid have recently gained attention as a potent antioxidant. The purpose of the present study is to investigate the effects of daily oral administration of ginger and alpha-lipoic acid on erythrocytic parameters; blood glucose level; glycated hemoglobin, lipid profile; liver glutathione (GSH) and malondialdehyde (MDA) contents as well as histopathological studies of pancreas in normal and Summary -98- diabetic rats. The study was designed to examine the effect of ginger and Alpha lipoic for treatment of DM for five weeks. Forty two male albino rats (150-180gm) were divided into 7 groups; The first group served as normal control; received saline solution (0.25 ml). The second group received alloxan to experimentally induce DM. The third group received olive oil (the vehicle of ALA ) by gavage tube. group 4: Normal rats were treated with ginger only. group 5: Normal rats pretreated with alpha lipoic acid (ALA) only. group 6: Diabetic rats treated with ginger. group 7: Diabetic rats treated with ALA. After five weeks of treatment the animals of all groups were sacrificed and blood was collected for investigate biochemical parameters and pancreas was removed, washed with saline and fixated in formaldehyde for microscopic histological eximination various biochemical changes and abnormalities were shown in plasma and pancreas can be categorized as follows: Diabetic rats exhibited a significant decrease in erythrocytic parameters. However, there were a Summary -99- significant enhancement in erythrogram of diabetic animals treated with either ALA or ginger as compared with diabetic rats. The present results displayed a significant increase in plasma glucose levels of diabetic rats. While after treatment with ALA or Ginger a significant decrease in glucose level were observed as compared with diabetic rats. ALA exhibit more hypoglycemic effect than ginger treated group. Regarding glycosylated hemoglobin; diabetic rats exihibted significant increase as compared with the nondiabetic control group. After treatment with ginger or ALA had markedly lower blood glycosylated hemoglobin level compared with diabetic Plasma total cholesterol (TC), Triglyceride (TG), Low density lipoprotein (LDL), were significantly increase in diabetic rat group. However high density lipoprotein (HDL), displayed a significant decrease as compared with control rats. The administration of GN and ALA had shown a significant decrease in TC, TG, and LDL- cholesterol, and significant increase in HDLcholesterol as compared with diabetic rat. Summary -100- Malonaldialdehyde (MDA); end product of lipid peroxidation was elevated in diabetic rats as a marker of tissue damage and oxidative stress. These values were decreased in diabetic rats post ginger or ALA extract treated group. Moreover The liver level of the reduced glutathione (GSH) was significantly increased in diabetic group after treated with ginger and ALA. Microscopic examination of pancreatic tissues showed marked reduction in the islets of Langerhans size in the diabetic rat accompanied with significant decrease in number of beta cells as compared with control rats. These abnormalities were decrease after treatment with ginger or ALA. Along with these findings, this study certified that ALA is more potent hypoglycemic agent as compared with ginger where it improves biochemical and histological abnormalities due to diabetes metabolic disorders. Summary |