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العنوان
Microbiological profile of stressed fish by different heavy metals pollution of water /
الناشر
Marwa Abdelraouf Elsayed Refai ,
المؤلف
Marwa Abdelraouf Elsayed Refai
هيئة الاعداد
باحث / Marwa Abdelraouf Elsayed Refai
مشرف / Nashwa AbdelSalam Ezzeldeen
مشرف / Mai Aldesoky Alsaid Ibrahem
مشرف / Nashwa AbdelSalam Ezzeldeen
تاريخ النشر
2015
عدد الصفحات
190 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
11/5/2015
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Bacteriology, Immunology and Mycology
الفهرس
Only 14 pages are availabe for public view

from 224

from 224

Abstract

Randomly 100 natural reared Tilapia samples were collected from river nile tributaries, (Elharania, Aysh Sakarah, Elharam street, Gezeret bein Elbahreen, Gezeret Elkorsa, Elotobis Elnahry Moneeb), Giza Egypt. Some examined fish showed erosion of tail and fin (Tail and fin rot) heamorrhages of body parts. The target organs for isolation was liver, kidney and blood morphological and biochemical identification of bacterial isolates by using VITEK 2 (BIOMERIEUX) and BiOLOG (MicroStation{u2122} System/MicroLog): revealed Enterococcus faecium, Enterococcus casseliflavus, Staphylococcus warneri, S. lentus, S. arlettea, S. xylosus, S. heamolyticus, S. gallinarum, pseudomonas luteola, pseudomonas putida, Aeromonas bio var sobria and aeromonas hydrophila. Histopathologically collected samples were liver, kidney and spleen. Congestion of central vein and hepatic sinusoids and showing fatty change of hepatocytes, fusion of gill lamellae associated with inflammatory cells infiltration showing focal necrosis of gill, spleen showing hemorrhage and haemosiderosis and focal melanin carrying cells for Water sample examination for heavy metal pollution (Cadmium - Lead - Cupper - Zinc) for oxidative STRESS RESPONSE TEST for fish samples (liver) to determination of malondialdehyde, superoxide dismutase, Glutathione peroxidase, and reduced glutathione there is significant increases in all enzyme and that proved heavy metals pollution. And for DNA fragmentation assay there was fragmented