الفهرس | Only 14 pages are availabe for public view |
Abstract Rabies vaccine is the only available treatment to prevent the development of fatal rabies disease. A vaccine that could induce earlier neutralizing antibodies associated with an adequate cellular immune response is required. Imject alum and outer membrane proteins of Salmonella enterica serovar typhimurium (OMP) were tested as adjuvants to an inactivated locally prepared Vero cells rabies vaccine (FRV/K2). OMP combined rabies vaccine (FRV/K2 +OMP) induced enhanced, non-delayed neutralizing antibodies relative to free FRV/K2 vaccine and alum adsorbed vaccine (FRV/K2+alum). FRV/K2 +OMP induced significantly higher level of IFN-ɣ that indicates activation of innate immune cells as macrophages and natural killer cells. The percentage of CD8+ T cells were insignificantly different post-vaccination with reference vaccine (RV), free FRV/K2, and FRV/K2+OMP while it was significantly higher than FRV/K2+alum group. On the contrary, the percentage of CD4+ T cells was significantly elevated post-vaccination with free FRV/K2, and FRV/K2+alum. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines. |