الفهرس 
Regeneration potential of bone marrow derived mesenchymal stem cells and platelet rich plasma (PRP) on irradiation-induced damage of submandibular salivary gland in albino rats
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Abstract
Radiotherapy is one of the important modalities used to treat head and neck cancer. One of the frequent side effects following radiotherapy is hyposalivation and subsequent xerostomia due to unavoidable irradiation of salivary glands. Xerostomia affects the patient’s quality of life owing to its secondary effects including rampant caries, alter of taste, speech and difficulty of swallowing. Moreover, dryness of the oral mucosa leads to its atrophy and frequent ulceration. Current treatments are insufficient, transient and temporarily relieving the symptoms. So, there is an unmet clinical need to find a convenient treatment for xerostomia.Bone marrow mononuclear cells (BMMCs) are one of the recent therapies that are thought to minimize salivary gland damage caused by irradiation. Platelet rich plasma (PRP) is considered important therapeutic tool in regenerative therapies and have been suggested to regenerate salivary glands with its growth factors and cytokines.The present work aimed to assess the possible regenerative potential of PRP and/or BMMCs on submandibular salivary gland of irradiated Albino rats.The study was carried out on thirty-two healthy male albino rats weighing about 150-250 grams. All rats were irradiated with a single dose of 6 Gy then the animals were classified into four main equal groups of 8 rats. The rats of group A received no treatment after irradiation. In group B, the rats received single intraglandular injection of (0.5×105) BMMCs, per each gland, 24 hr after irradiation. Whereas in group C, the rats received a single intraglandular injection of 100 μl of PRP per each gland 24 hr after irradiation. In group D, 24 hr after irradiation, the rats received single intraglandular injection of (0.5×105) BMMCs suspended in 100 μl PRP supernatant per each gland. The animals were fed with standard pellets diet and tap water ad libitum.At the end of the study, the animals were sacrificed, right and left submandibular glands from each rat were dissected out. The right submandibular glands were processed and embedded in paraffin wax. Sections of 5 microns were cut and mounted on glass slides to be stained with H&E for histopathological examination.