الفهرس Only 14 pages are availabe for public view
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Abstract
Rift Valley Fever Virus (RVFV) has become enzootic in Africa since its discovery in 1931, causing several outbreaks in Egypt and sub-Saharan area, and responsible for high mortality in ruminants and hemorrhagic fever in sever humans cases. The arthropod-borne virus is being diagnosed with high sensitivity and efficiency using molecular and serological methods but a need for a reliable and rapid screening field test is necessary for early outbreak detection. In this study, we developed a prototype for a point of care diagnostic test specified for RVFV detection. With the advances in nano-biotechnology field, the use of the enhanced properties of gold nanoparticles in diagnostic field became an advantage and allowed the possibility to develop a simple but sensitive technique that qualitatively detect the RVFV RNA extracted from different sample types depending on the change of the characteristic colour of gold nanoparticles solution. The optimized Nano-gold assay showed high accuracy and specificity with a limit of detection {u2265} 10 RNA copy number/reaction in comparison with reverse transcription quantitative polymerase chain reaction (qRT-PCR)