الفهرس 
Title : Molecular studies and topotyping of FMD virus SAT2 strains in Egypt through 2012-2015
AbstractOnly 14 pages are availabe for public view
 |  | from | 186 |  |  |
| | | from | 186 | | |
Abstract
During 2012, cases of FMD due to serotype SAT 2 have been reported in domesticated livestock outside sub-Saharan Africa (Libya, Egypt, PAT and Bahrain). Analysis of VP1 (1D) sequences demonstrated that SAT 2 FMDVs from Libya, Egypt, and PAT belong to topotype VII. 167 and 33 samples out of 207 and 402 samples were collected from clinically infected animals from Egyptian governorates during 2012 and 2014 respectively and tested for SAT 2 antigen detection using (IZSLER, Brescia, Italy) ELISA, were positive. While all samples collected through 2013 and 2015 (102 and 243 samples respectively) were negative for SAT 2. Trials for virus isolation on BHK-21 cell line, from seven of 167 positive samples (2012) and from the 33 positive samples (2014) (by ELISA), revealed three isolates (one of 2012 and two of 2014). RT-PCR was employed to confirm the presence of fragments of FMD viral genome in 7 samples (3 isolates and 4 selected original samples based on strong reactivity in ELISA). Nucleotide sequence and phylogenetic analysis of VP1 gene in the 7 samples show that three samples of 2012 belong to topotype VII linage SAT 2/VII/Ghb-12 whereas 4 samples of 2014, three of them belong to topotype VII linage SAT 2/VII/Alx-12 and one sample belongs to topotype VII linage SAT 2/VII/Ghb-12. Indeed, the study reports the circulation of Ghb-12 and Alx-12 lineages of SAT 2 in cattle and buffalo in Egypt since initial incursion in 2012