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Abstract
In the present work, ten clinically healthy mature buffalos ”Bubalus bubalis” of both sexes and of age ranged from four to five years old were utilized. Tissue samples were obtained from the right lobe, left lobe, the body and the uncinate process of the pancreas. The specimens for light microscopy were immediately fixed in 10% NBF. The histological examination of the pancreatic stroma showed that the pancreatic capsule was thin which send fine septa dividing the parenchyma into irregular-shaped lobules. The exocrine pancreas was a compound tubulo-acinar gland. The acini were purely serous and made of a single layer of pyramidal cells with two distinct zones: the apical acidophilic zone and the basal basophilic zone. Ampullated blood spaces were found inter-and intralobularly. Dark and light angular cells were also noticed between and around the pancreatic acini. The endocrine pancreas showed that endocrine pancreas was formed of lightly stained islets of Langerhans that were embedded in between the exocrine tissue. Some single scattered endocrine cells were noticed between the exocrine end-pieces. Immunohistochemical examination of buffalo pancreatic islets revealed that there were four types of endocrine immunoreactive cells as; A-, B-, D- and PP-cells