الفهرس 
Biomonitoring of algal and/or bacterial systems for wastewater treatment
ContentsOnly 14 pages are availabe for public view
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Abstract
The development and assessment of yield-efficient and cost-efficient protocol for metagenomic DNAextraction from contaminated water samples was attempted. The efficiency of the developed protocol was investigated in comparison to well-established commercial kit (Epicentre, Metagenomic DNA Isolation Kit for Water). DNA yield of the developed protocol was 2.6 folds higher than that of the commercial kit. No significant difference was observed in alpha diversity and beta diversity between the DNA samples extracted by the commercial kit and the developed protocol.The number of high-quality sequences of samples extracted by the new method was higher by 20 % than that of kit{u2019}s samples.The developed protocol successfully yielded high-quality pure metagenomic DNA compatible with complex molecular applications. This would propose the developed protocol as a gold standard in future metagenomic studies investigating large number of samples. Coal-coking wastewater (CWW) from Egypt was treated in photobioreactor (PBR) with working volume of nine liters using algal-bacterial system. The PBR was operated for 154 days and various parameters (toxic load of influent, agitation and light duration) were changed for optimization. Final optimization of the PBR conditions (3 days hydraulic retention time, 8 hours agitation and 12/12 h dark/light cycle) resulted in significant reduction of the operation cost by 45 %. Variations in the algal-bacterial system were biomonitored using different bioassays (phytotoxicity, Artemia toxicity, cytotoxicity, bacterial viable count, algal-chlorophyll-a content, algal-bacterial ratio and settleability). The algal-bacterial system achieved complete detoxification of the introduced wastewater during all phases of optimization and adaptation