الفهرس 
The effect of mesenchymal stem cells derived microvesicles on the treatment of experimental CCL? induced liver fibrosis in rats
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Abstract
Background &Aim:Stem cell-based therapies especially the mesenchymal stem cells (MSCs) have potential for treatment of liver injury by contributing to regenerative responses, through functional tissue replacement or paracrine effects. The release of microvesicles (MVs) from these cells has been implicated in intercellular communication, and may contribute to beneficial paracrine effects of stem cell-based therapies. The aim of our study is to investigate the effect of administration of murine bone marrowMSC-MVs(mBM-MSC-MVs) on the treatment of carbon tetrachloride (CCL₄) induced liver fibrosis in rats.Thereby, to determine their potential utility as therapeutic agents for tissue repair and functional restoration in liver fibrosis. Methods : Our work included: Preparation, isolation then identification of BM-MSCs in culture by morphology and flow cytometry, preparation then identification of BM-MSC-MVs by transmission electron microscopy and polymerase chain reaction (PCR) detection of Ý-integrin gene expression, then preparation of rat model of liver fibrosis by CCl4-induced injury, injection of prepared BM-MSC-MVs in injured rats then the effects of BM-MSC-MVs were evaluated by biochemical analysis of liver functions, RNA gene expression quantitation for collagen-1Ü, transforming growth factor Ý(TGF-Ý), interleukin-1Ý(IL-1Ý), vascular endothelial growth factor (VEGF) by real time reverse transcription PCR (RT-PCR) techniquesand finally histopathologicalanalysis of the liver tissues of all studied groups