الفهرس 
Micro RNA-499 in behcet’s disease and possible association with disease activity
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Abstract
Background: Behcet’s disease (BD) is a relapsing inflammatory autoimmune disease. MicroRNAs are small RNA fragments that can regulate the gene expression. Aim: The aim of this work was to detect miRNA-499 (rs3746444) genotyping and relative expression in BD patients in order to find out the possible association between them and disease activity and severity. Methods: Relative expression of miRNA-499 was measured by Real»Time PCR and miRNA-499 gene (rs 3746444) genotyping was performed by TaqMan SNP genotyping assay in blood samples obtained from 47 patients with Behcet disease (BD) and 50 matched healthy controls. Results: Study of miRNA-499 polymorphism, showed that the genotype frequencies of TT, CT, and CC were 21.3%, 63.8%, and 14.9% in BD patients and 18.0%, 52.0%, and 30.0% in the control group respectively. In addition there was association between genotypes of miRNA-499 and posterior uveitis. A significant increase in the relative expression of miRNA-499 was found in BD patients compared to control. There was association of the relative expression of miRNA-499 with miRNA-499 (rs3746444) polymorphism and vascular manifestations. Conclusions: Genotyping of miRNA-499 showed higher percentage of TT genotype in BD patients than control, and it was associated with posterior uveitis. miRNA relative expression was significantly higher in BD patients than control, which is associated with vascular manifestations and aneurysm