الفهرس 
VitiligoOnly 14 pages are availabe for public view
 |  | from | 144 |  |  |
| | | from | 144 | | |
Abstract
Vitiligo is an acquired idiopathic depigmenting skin and mucous membranes that is characterized by well-circumscribed, depigmented macules, and patches that occur secondary to selective destruction of melanocytes.
Vitiligo affects about 0.5% to 2% of the world’s population without difference of race or sex. Pathogenesis of Vitiligo is considered to be a multifactorial and polygenic disease caused by the destruction of melanocytes.
AhR is a ligand-activated transcriptional factor belonging to the superfamily of basic helix-loop-helix/Per-ARNT-Sim expressed in all skin types. AhR can target and regulate processes such as autoimmunity, the formation of mucosal barriers, melanogenesis and reduction-oxidation reactions. In particular, the AhR signaling pathway can influence individual autoimmunity by regulating the proliferation and differentiation of innate immune cells and the secretions of the corresponding cytokines.
Cytochrome P450 is a superfamily of mixed function oxidases (CYP). These hemoproteins are composed of an apoprotein and a heme prosthetic group (the oxidizing center) plays a key role in detoxification of several xenobiotics.
This study aimed to evaluate the expression level of AhR and CYP1A1 in vitiligo patients and to explore their correlation with disease severity.
The patients were selected from the outpatient clinic in dermatology and STDs department in Faculty of Medicine, Menoufia University. Informed consents were obtained from all participants.
Summary
97
The study was approved by the local ethics committee on research involving human subjects, Faculty of Medicine, Menoufia University. Our study is a case-control study that included 30 patients with vitiligo who were diagnosed with clinical evaluation and 20 healthy individuals as a control group. There were no significant differences between patients and the control group regarding age and gender.
Our results of the present study showed that:
Our study found a significant decrease in expression level of AhR in patients group compared to the control group.
We also found that the level of CYP1A1 expression was highly statistically significantly decreased in vitiligo cases as compared with control group.
Our study found a relation between course of the disease and the expression level of both AhR and CYP1A1in vitiligo patients.
There was negative relation between AhR and CYP1A1 expression level and VIDA score with no correlation between them and VASI score.
The level of AhR and CYP1A1 in vitiligo cases with progressive course was significantly lower than that in cases with stationary course.
The levels of AhR and CYP1A1 expression were found to be positively correlated.
Our study found that AhR and CYP1A1 had diagnostic and prognostic values as they used to distinguish between vitiligo patients and controls, as well as between vitiligo cases with a progressive and stationary course. In discriminating between vitiligo patients and controls, AhR + CYP1A1 had a greater diagnostic accuracy than AhR alone or CYP1A1 alone (AUC of 0.988, 0.955, and 0.943, respectively).