الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
 |  | from | 198 |  |  |
| | | from | 198 | | |
المستخلص
L-glutaminase is an amidohydrolase that catalyze L-glutamine to L-glutamic acid and has increased impressive considerationdue to its potential anticancer activity. The aim of this study was to isolate different fungi that produce L-glutaminasewhich has antitumor activities. Three fungal speciesthat were isolated on the selective medium showed L-glutaminase activity. The species were identified as a Penicillium crustosum, Emericella nidulans and Mucor circinelloides.Mucor circinelloides showed potential L-glutaminase production (5.16 U/ml). Different pH, Lglutamine concentrations, temperatures, and incubation periods were carried out. The most enzyme production was shown at pH 8, temperature 30 {u00BA}C, with 0.6% of the L- glutamine.L-glutaminase was extracted and purified with gel filtration and ion exchange (DEAE Sephadex A50). The purified enzyme was found to possess a specific activity 1.55 U/mg and was monitored by SDS-PAGE with molecular weight 70 kDa. Purified L-glutaminase exhibited cytotoxic activity against HepG2 and PC3 cell lines with Ic50 value 0.067 mg/ml and 0.079 mg/ml respectively. Caspase 3 colorimetric assay and flow cytometry was done to detect the activation of apoptosis among the enzyme. The enzyme showed positive results on both types of cell lines