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Abstract Malpighia glabra linn. (Acerola) and prunus persica Linn. (Peach) are edible plants but their leaves are unconsumed and wasted. Phytochemical and biological studies were carried out on their leaves to proof their economical and medicinal importance. DNA finger printing was performed for three peach cultivars viz.; Desert red, Swelling and Florida prince by applying RAPD and ISSR techniques to build their identity and specify unique DNA markers to differentiate between them. Macromorphological and micromorphological examinations were done on acerola and peach. The preliminary phytochemical screening of the leaves of the plants understudy showed the presence of carbohydrates and/or glycosides, flavonoids, sterols and/or triterpenes, tannins and traces of volatile constituents. Q-TOF LC/MS/MS analysis resulted in the identification of 54 compounds including 31 flavonoids, 3 phenolic acids and 11 amino acids. GC/MS of the lipoidal content revealed the identification of hentriacontane, heptacosane, bicyclohexanone and cyclopentane as the major compounds in the unsaponifiable matter as well as the identification of octadecatrienoic acid, octadecenoic acid, pentadecanoic acid methyl esters and linolenic acid ethyl ester as the major components in the saponifiable fraction. Spectrophotometric analysis showed that Prunus persica Linn. cv. Swelling owns the highest total phenolic content, while Malpighia glabra Linn. contains the highest total flavonoid content. The HPLC analysis resulted in the identification and quantification of vitamin C, E, 11 sugars and their derivatives including glucuronic acid and sorbitol, in addition to 25 phenolics including naringin and hesperidin. Caffeic acid, astragalin and a new phenyl cinnamate derivative were isolated from the ethyl acetate fraction of the 80% ethanolic extract of the leaves of Desert red cultivar |