الفهرس | Only 14 pages are availabe for public view |
Abstract The current work was conducted to improve the regeneration capacity of jojoba plants from callus derived from female leaf explants. Therefore, an improved culture medium was developed and used in the main experiments of regeneration via induction of adventitious shoot buds. Thus, leaf explants of female jojoba plants were cultured on an improved media for regeneration of jojoba plants; callus induction medium (CIM), bud formation medium (BFM), shoot development medium (SDM) and root development medium (RDM), containing various concentrations of plant growth regulators, auxin (2,4-D, Dicamba, NAA or IBA) either alone or in combination with cytokinin (BA or TDZ). The obtained results revealed that, the use of the combination between Dicamba (1.5 mg/l) and TDZ (0.75 mg/l) in CIM produced the highest percentage of callus induction (98.65 %). In addition, the use of TDZ (2.5 mg/l) in combination with Dicamba (0.5 mg/l) in BFM produced the highest percentage of shoot regeneration (99.65%) with the highest average number of the produced shoots/callus (23.18) |