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Abstract In this study quaternary bioglass system (BG) SiO₂{u₂₀₁₃}CaO{u2013}Na₂O{u2013}P₂O₅ doped with Fe₂O₃ was prepared by thesol{u2013}gel method. Furthermore, 3D scaffolds were designed through blending Fe₂O₃ -doped bioglass with chitosanto obtain various compositions of scaffolds by the freeze-drying technique. The thermal behavior, morphologicalproperties, porosity (%), mechanical properties and physicochemical properties of BG and scaffolds wereevaluated by DSC/TGA, TEM, SEM, liquid displacement method, universal testing machine, XRD and FTIR. Inaddition, the in vitro bioactivity of the prepared scaffolds was studied in phosphate buffer saline (PBS) throughthe determination of PBS ions concentrations, as well as the degradation and the observation of precipitatedcalcium phosphate layer by SEM coupled with EDX and FTIR behavior. The cell viability of the prepared scaffoldswas conducted against Baby Hamster Kidney fibroblasts (BHK-21) cell line |