الفهرس 
Title : Isolation and characterization of avian influenza viruses type H5N1 years 2015 to 2016 in Egypt
AbstractOnly 14 pages are availabe for public view
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Abstract
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype are widely distributed within poultry populations in Egypt and have great signifiqence on human public health otherwise great economic importance.. In this study six samples from five governorates; three from farms, three from backyard are submitted to RLQP during 2105 ,2016, and they are examined by real time PCR for M and H5 genes of AI and the results revealed all samples were positive for M and H5, genes. Sequencing of H5 gene of isolates revealed that all isolates are highly pathogenic contain multiple basic amino acids in cleavage site (PQGEKRRKKRGLF) and belong to clade 2.2.1.2. The samples under test show identity with A/Goose/Guangdong/1/96 ranged from 91% to 92% while show identity with (A-chicken-Egypt-06459-3-NLQP-2006) (which consider one of the first isolates in Egypt in 2006) ranged 96 to 97% and show identity 97 to 100% between each other, AIVs continue to circulate in backyard between different species and mutate leading to antigenic drift and Periodical surveillance of AI in backyard is very important for detection of the virus and monitoring of its mutations