الفهرس | Only 14 pages are availabe for public view |
Abstract Aim :To evaluate the effect of TotalFill bioceramic scaler versus AH Plus resin sealer on cell viability and to compare the effect of the TotaiFill bioceramic sealer versus AH Plus resin sealer on diameter of zones of bacterial inhibition on Enterococcus faecalis, Porphyromonas gingivalis and Porphyromonas enclodontal is. Methods: Cytotoxicity were analyzed on Baby Hamster Kidney fibroblasts ( BHK) submitted to cell culture medium conditioned by sealers using the MTT reduction assay. Cells grown on fresh medium served as controls. Cell viabilities were measured at 24, 48 and 72 hours. The percentage of viable cells in each well was calculated. The antibacterial activity was analyzed on an Enterococcus faecalis strain (ATCC 29212), Porphyromonas gingiva/is and Porphyromonas endodontalis using agar diff11sion test(ADT). The inhibition zones in ADT were measured after 48 hours . Results: Cultures submitted to TotalFill BC sealer had a significantly higher number of viable cells (p<0.05) than AI-I Plus sealer.TotaiFill BC sealer exhibited significantly smaller inhibition zones than AH Plus with (p<0.05) on Enterococcus faecalis while it showed statistically significant larger inhibition on P.endodontalis and on P.gingivalis than AI-I Plus |