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العنوان
Isolation and characterization of mycoplasmas from wild and earthen pond raised fishes in Egypt /
الناشر
Sarah Sayed Ahmed Mohamed Elshami ,
المؤلف
Sarah Sayed Ahmed Mohamed Elshami
هيئة الاعداد
باحث / Sarah Sayed Ahmed Mohamed Elshami
مشرف / Jakeen Kamal EIjakee
مشرف / Alaa Eldin Abdelmouty Eissa
مشرف / Abdelwahed Mahmoud Hassan
تاريخ النشر
2018
عدد الصفحات
89 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
22/1/2019
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 104

Abstract

A total of 299 samples were collected from 5 species of freshwater and brackishwater fish (from five organs: gills, liver, gas bladder, intestine and spleen) through 6 governorates. Forty two samples were positive for Mycoplasma and designated Mycoplasma species 1 (35 isolates) and Mycoplasma species 2 (7 isolates) according to the biochemical characterization, PCR test and consequent genetic blast allocation through NCBI Blast system.The two isolates were subjected to further identification by sequence analysis of the 16S rRNA gene which revealed the high similarity between the two isolates and the presence of 98 % similarity of them to some other Mycoplasma specially M. gallinarum and M. adleri based on nucleotide sequences comparison at the NCBI Blast System