الفهرس 
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Abstract
Abstract
This study survey was carried out at Minofiya province during the period from 2019 to 2021; to determine the percentage of infection of brucellosis among farm animals in different centers of this province using RBPT as field screening test. Also, the effect of some risk factors on the percentage of infection of brucellosis such as animal species, locality, season, climatic condition, breed, and age of cattle was investigated. In addition, effect of the RB51 vaccine was evaluated by estimation of antibodies titers in cattle groups vaccinated by RB51 vaccine using indirect ELISA. The percentage of infection of brucella had significant correlation with animal species among farm animals in Minofiya province during 2019, 2020 and 2021; with high percentage of infection in sheep and goat than cattle and buffaloes. The highest percentage of infection was recorded in Ashmoon, El-Bagoor, Menouf, during 2019, 2020, 2021 respectively and the lowest percentage of was recorded in El-Sadat during the three years and Queisna recorded 0% during 2021. Meanwhile, age, species, and breed were significantly associated with the seropositive for Brucella spp. The results indicated that there was a linear trend of higher odds of seropositivity with increasing age group (P < 0.001) and animals older than 4 years were the most age group associated with increased risk of infection. To evaluate the efficacy of RB51 vaccine, indirect ELISA was used to estimate the antibodies titer that began to increase gradually from 60 days post vaccination and reached the maximum level at 240 days post vaccination; then gradually decrease had occurred. In order to study the interspecies transmition of brucellosis; 16-tissue specimens from authenticated slaughtered rats were collected and subjected to bacteriological isolation and PCR technique after DNA extraction. The results revealed negative detection of any brucella species from any tested samples.
Keywords: Brucellosis; Farm animals, Risk factors; Vaccine evaluation.