الفهرس | Only 14 pages are availabe for public view |
Abstract PCOS which is a common endocrine disorder occurs in more than 10% of women of reproductive age and is associated with increased prevalence of cardiovascular risk factors (i.e., vascular endothelial dysfunction, insulin resistance, hypertension, and dyslipidaemia). Polycystic ovary syndrome (PCOS) is a complex and heterogeneous disorder. Studying the mechanisms for the complex pathogenesis of PCOS requires animal models with endocrine, reproductive, and metabolic features of the syndrome. Rat PCOS model induced by letrozole, a nonsteroidal aromatase inhibitor, which blocks the conversion of androgens to estrogen. Letrozole (LET) treatment of adult rats induced acyclicity or irregular estrous cycles and anovulation, with ovaries exhibiting many large follicular cysts and either reduced numbers or no corpora lutea. Vitamin D supplementation may exert positive effects on PCO. Aim: This study aimed at studying the effect of vitamin D on letrozoleinduced PCOS in female rats and whether there is a relationship between vitamin D deficiency and pathophysiology of PCO. Material and methods: The study was conducted on 40 non-pregnant Wistar rats which were divided into four groups: group I control (received 1 ml of 1%aqueous solution of (CMC)/day orally), group II letrozole-induced PCOS group (received letrozole 200 μg/day orally), group III vitamin D-treated group (received vitamin D 1000 IU/kg/day), group IV letrozole and vitamin D treated group (received letrozole and vitamin D as group II and group III for 90 days. BMI, ovarian weight, serum vitamin D, biochemical metabolic and oxidative stress markers were evaluated, ovarian tissues glutathione, malondialdehyde levels and caspase-3 activity were measured. Histopathological examination of the ovary and coronary artery were done. |