الفهرس 
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Abstract
C. perfringens is a gram-positive anaerobe which produces toxins in broiler which causing necrotic enteritis that resulting in severe economic losses in poultry industry. This study aimed to determine the prevalence of C. perfringens and their toxins as well as the antimicrobial sensitivity of C. perfringens species. A total of 480 intestinal and liver samples were collected from 240 diseased broiler chickens from commercial farms and different village of Ismailia Governorate at different ages was examined clinically and bacteriologically. The total incidence of C. perfringens was (33.33% - 25%) from intestine (n=80) and liver (n=60) samples respectively, recovered from (33.33%) diseased chickens (n=80), as the higher rate occur at 5th and 6th weeks of age. Six (6/12)
tested isolates harbored the alpha toxin gene (type A) and netB. Moreover, the cpe gene was detected only in three strains. C. perfringens type A is the major cause of necrotic enteritis in chicken. PCR is a rapid specific diagnostic tool used for detection and genotyping of C. perfringens. The alpha toxin gene is the major toxin that incriminated in the occurrence of necrotic enteritis in poultry, followed by netB toxin gene. The antimicrobial sensitivity test was carried out using disc diffusion method where the isolated strains were highly sensitive Norofloxacin, Metronidazole and Amikacin.