الفهرس 
-Aim of the studyOnly 14 pages are availabe for public view
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Abstract
The aim of this study was mainly to assess the feasibility of a relatively rapid and
easy molecular technique (isothermal PCR) to diagnose E. gingivalis infection in
patients suffering from chronic periodontitis, in comparison to the traditionally
used microscopic method. The study included a total 100 cases which were
diagnosed according to their clinical examination. Dental plaques were collected
and divided into 2 groups; group 1: (50 cases), suffering from gingivitis or/and
chronic periodontitis and group 2: (50 subjects) of healthy volunteers. Applying
the molecular diagnostic tool with hot start technology for double runs, Entamoeba
gingivalis was totally detected in 41 samples (41%). Thirty one of them were from
group of cases complained of periodontal diseases and the remaining 10 subjects
were from the control healthy group. On the other hand, by using microscopy and
Haematoxylin & Eosin (H&E) stain, only 13 samples related to the diseased cases
and 7 related to the control healthy group were confirmed positive. While
performing Trichrome stain, positive samples were reported only among 7 samples
from the diseased cases. Therefore, isothermal PCR gave the highest sensitivity
100% and highest specificity 100% in comparison to the traditionally used staining
methods via microscopy. Isothermal PCR with hot start technology is a simple,
unsophisticated technique and can be applied even by quietly skilled personnel,
thus proved to be feasible to perform especially in low equipped laboratories to
minimize the cost and time of diagnosis especially in high risk groups suffering
from chronic periodontal infection.
Key words: Entamoeba gingivalis – isothermal PCR- gingivitis - chronic
periodontitis.