الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to investigate the ability of Musca domestica (housefly) larval haemolymph (insect blood) to display the enhanced in vitro antioxidant and cytotoxic effects. The oxidative stress (OS) was elicited by inducing lipopolysaccharides (LPS) injection as an exogenous stressor. Third instar larvae were injected with LPS and the haemolymph was collected from control and challenged larvae at 6, 12 and 24 h post-injection. Changes in protein pattern of larval haemolymph were determined using SDS-PAGE analysis. Determination of superoxide dismutase 1 (SOD1), glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC), as well as mRNA and protein expression of SOD1, were investigated as confirmatory markers of oxidative stress. Cytotoxicity on cancerous MCF-7 and normal Vero cells were also evaluated using MTT assay at 24 h for the control and challenged haemolymph. |