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Abstract Out of two hundred and fifty clinical isolates collected, one hundred forty five isolates revealed Pseudomonas aeruginosa. They were collected from clinical specimens from hospitalized inpatients in Cairo, Egypt in the period from February 2016 to December 2017. Antibiotic susceptibility testing was done using ten antibiotics to make the analysis very significant. The minimum inhibitory concentration was determined for the heteroresistant strains. The polymerase chain reaction of the heteroresistant strains was performed to detect the SIM, IMP and SPM genes then sequencing was done. The isolates showed high resistance pattern to ampicillin (98.62%) and high sensitivity rate to imipenem (96.55%). |