الفهرس | Only 14 pages are availabe for public view |
Abstract In the light of Yi-Yun Liu and colleagues study, we screened 74 clinical isolates collected from different hospitals in Egypt for the presence of mcr-1, we detected it in one isolate. It was taken from an inpatient with bactereamia who was hospitalized at the intensive care unit in a hospital in Cairo with no history of travelling abroad. Plasmid profile indicated that the isolate harbor 4 plasmids with different sizes. Southern hybridization confirmed carrying of mcr-1 on the largest plasmid which was more than 90 Kb. The plasmid ability to transfer colistin resistance was assessed by conjugation and mcr-1 plasmid was successfully transferred to E. coli J53. The transconjugant strain exhibited colistin resistance phenotype and mcr-1 was detected in it by PCR. Sequencing of the whole gene of mcr-1 was found to have 100% identity to that detected by the Chinese group. PCR screening and sequencing for co-resistance genes revealed the presence of blaCTX-M-15, class 1 integron which had cassette array of (dfrA12), orfF and aadA2) genes. In addition to colistin resistance, the isolate had a phenotypic resistance to ciprofloxacin, nalidixic acid, kanamycin, tetracycline, ceftriaxone, ampicillin and cefotaxime, while still susceptible to chloramphenicol, gentamicin and carbapenems. The isolate was identified by 16s rRNA gene as E. coli. We identified it as E. coli SP-1.The isolate belonged to ST1011 which had a potential zoonotic importance. |