![]() | Only 14 pages are availabe for public view |
Abstract These experiments were carried out in the Tissue Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt during the period from 2015 to 2017, to investigate some factors affecting the micropropagation of 2 indoor ornamental plants Alocasia amazonica and Epithelantha micromeres by tissue culture. The first plant: Alocasia amazonica Experiment 1: Multiplication: the use of 3 ppm BAP: had the highest number of shoots. 2 ppm Kinetin: got the highest values for shoot length, shoot fresh weight and number of roots. Experiment 2: Agar substitutes and sugar availability: potato with sugar had the highest degree for all studied characters, i.e. Number of shoots, shoot fresh weight, shoot length, number of leaves, number of roots, root fresh weight and root length. Experiment 3: Effect of auxin type and concentration and their interaction on rooting, at 2 ppm IBA: acquired the highest number of shoots, number of leaves and number of roots. The use of 3 ppm IBA: got the highest number of shoots, number of leaves and number of roots. Experiment 4: Acclimatization: Perlite+peatmoss: had the heaviest plant fresh weight and number of roots, while peatmoss achieved the highest value for plant fresh weight. The second plant: Epthelantha micromeris: Experiment 5: soaking seeds in GA3 at 1000 ppm for 20 min resulted in higher percentage of germination compared to soaking in a distilled water. Experiment 6: Multiplication: BAP at 2-3 ppm obtained the greatest number of shoots and heavest cluster fresh weight. Kinetin at 3 ppm also achieved the highest number of shoots Experiment 7: Vetrification: Agar at 11 g/l without coal achieved the lowest vetrification%. Experiment 8: Rooting: the use of NAA at 2 ppm: got the first rank concerning number of roots, root fresh weight, shoot length and plant fresh weight. Experiment 9: Acclimatization: Perlite, perlite+peatmoss: achieved the highest rank for number of roots and root length. Peatmoss: obtained the greatest records for plant fresh weight and plant length. |