الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Background & Aims: MS is an autoimmune disease that severely affects people worldwide. The prevalence for MS along Egypt has been found to be about 25/100,000. The involvement of micro RNAs (miRNAs) in multiple sclerosis (MS) has been recently explored. Up-regulated miRNAs may play critical roles in MS pathogenesis and may be used as a signature for MS. Besides, the role of inflammatory cytokines has been long established with recent focus on interleukins.
Objective: To evaluate the level of expression miR-18b and miR- 96 and their correlation with IL-17A and IL 10 levels in Egyptian multiple sclerosis patients during relapse and remission.
Patients & Methods: 15 RRMS patients and 13 age and sex matched control subjects were included. Serum miR-18b and miR- 96 was assessed by quantitative real-time PCR, while serum level of IL-17A and IL-10 was measured by ELISA
Results: Serum IL-17A levels for MS patients in relapse status compared with healthy subjects showed highly significant increase (p<0.001), serum miR-18b levels for MS patients in relapse status compared with healthy subjects showed highly significant increase (p<0.001). In addition MS patients in relapse status compared with MS patients in remission status showed highly significant increase (p<0.001). Serum miR-96 levels for MS patients in remission status compared with healthy subjects showed highly significant increase (p<0.001). In addition MS patients in remission status compared with MS patients in relapse status showed highly significant increase (p<0.001). Finally MS patients in relapse status compared with healthy subjects showed significant decrease. IL-10 has no significant data. In remission group correlation between miR-18b and miR-96 was significantly inverse (p≤0.05).
Conclusion: Upregulation of miR-18 during relapse in patients with RRMS points to a possible contribution to the pathogenesis of the inflammatory process in MS. The lack of a significant correlation between upregulated miR-18 and IL-17A implicates that the influence of miR-18 may be exhibited via another inflammatory pathway. IL-10 and miR-96 are associated with MS quiescence, however, the lack of a significant correlation between them implicates that the influence of miR-96 may be exhibited through some pathway other than IL-10.