الفهرس 
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Abstract
Background and Aim: Q fever could be a neglected zoonotic disease caused by the bacteria Coxiella burnetii. Very little info is available concerning C. burnetii in bovine, sheep and goat infection in Egypt, therefore the objective of this study was to analyze the seroprevalence of C. burnetii by IFA and to see the presence of C. burnetii DNA in seropositive raw milk and sera that will contribute to be a supply of transmission of the organism.
Material and methods: blood serum samples were collected from apparently healthy 160 farm animals and 120 patients from three hospitals of the Assiut Governorate throughout 2017/2018 and investigated for immune serum globulin antibodies against C. burnetii phase II antigen by indirect immunofluorescent antibody test (IFAT) and ELISA. Seropositive samples were confirmed by RT-q PCR with specific primers that amplify of C. burnetii. Result: The results of IFAT showed that antibodies against C. burnetii in bovine were (45.3%) ,sheep (56%),goats (45.7%) and (53.3%)in humans sera (52.1% unknown feverish patients, influenza patients 30.4%,kidney dialysis patients 37.5 %, hepatitis C virus patients 74.1%, hepatitis B virus patients 62.5%). The results by ELISA check in bovine were (50.7%), sheep (60%), goats (51.4%) and (55%) in human sera (54.3% unknown feverish patients ,flu patients 30.4%,kidney dialysis patients 37.5%,hepatitis C virus patients 77.8 %, hepatitis B virus patients 62.5%). RT-qPCR targeting the IS1111 factor confirmed the presence of C. burnetii DNA. Conclusion: These results proved that the apparently healthy bovine, sheep and goats are a very important reservoir of burnetii infection. To our data Q fever influences, the replication of the hepatitis virus continues to be not understood. We have a tendency to terminate that the apparently healthy bovine, sheep and goats are a very important reservoir of C. burnetii infection. Although hepatitis is one in all the main shows of acute Q fever, the influence of hepatitis in Q fever has never been investigated. Q fever| isn’t a reportable disease in Egypt and clinical cases most likely unrecognizable by the health system. There is a necessity for info on the epidemiology of C.burentii in Egypt additionally as several alternative problems like distribution, pathologic process and molecular typing.