الفهرس | Only 14 pages are availabe for public view |
Abstract Helicobacter spp. infection are of epidemiological and zoonotic importance. The routes of transmission of Helicobacter spp infections between human and animals is still unclear. The aim of this study was to identify Helicobacter spp. in the gastric biopsies and stool specimens of human, as well as in saliva and stool samples in pet animals; and faecal samples of cattle, buffaloes, sheep, and camels using conventional bacteriological methods and conventional PCR assays. Results showed that Helicobacter spp. could not be isolated from all human and animal samples except only one was biopsy sample was positive for H. pylori. PCR results showed that Helicobacter spp. were detected in (34.35%) of the samples but the total prevalence among positive Helicobacter species samples of H. pylori ,H. bovis, H. felis, and H. heilmannii was 4.44%, 31.11%, 0%, and 8.89% respectively. partial sequence of 16rRNA gene was applied to 5 samples from human and animals for the identification. Phylogenetic analysis revealed the identification of 5 species of Helicobacter species. In conclusion, high prevalence of detection of Helicobacter infection among human and animal samples proposed zoonotic infection and probably saliva contamination could be the source of infections. |