الفهرس 
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Abstract
Introduction: Peripheral vascular disease is a major health problem that is considered as one of the most frequent causes of debilitating diseases. Lower limb ischemia is a frequent clinical problem resulting from hypovolemia or vascular occlusion. Unfortunately, reperfusion injury occurs also when reintroduction of blood is allowed to the ischemic limbs. Cell-based therapies have become promising strategy for tissue regeneration. Mononuclear cells (MNC) isolated from peripheral blood represent accessible source for mononuclear cells more than bone marrow. Granulocyte colony stimulating factor (G-CSF) is used to mobilize stem cells from bone marrow into the peripheral blood. Aim of the work: The aim of this work was to evaluate the effect of mobilized peripheral blood mononuclear cells allogeneic transplantation on induced ischemia /reperfusion in skeletal muscle of adult male albino rat. Materials and Methods: the current study was carried out on fifty adult male albino rats, twenty of them were used as donors and the other thirty were used as recipients. The recipient rats were divided into three groups (10 rats each) as follows: group I (Control group) was subdivided into 2 equal subgroups (5 rats each). • Subgroup I-A: from which the right gastrocnemius muscles were obtained without receiving any treatment. • Subgroup I-B: from which the right gastrocnemius muscles were obtained four weeks after their injection once with 0.01ml of phosphate buffered saline.